n-3 LC-PUFA Enrichment Protocol for Red Earthworm, Eisenia fetida: A Cheap and Sustainable Method

This study assessed the potential of omega-3 long chain polyunsaturated fatty acids (n-3 LC-PUFA) enrichment in red earthworm (Eisenia fetida, REW), via addition fish oil (FO, anchovy oil) to culture-compost. FO compost was tested at graded inclusion doses (0, 2.5, 5 and 10 g/100 g compost) for different timeperiods, up 96h. Dose, time, as well dose time interactions had significant effects on all bodies. The with FO/100 sharply increased DHA levels worms (14.01 mol%) within just 24 h, compared control group (0.92 mol%). Similar increases (ranging between 10.99 15.55 occurred only after 48 96 h lower (2.5 5.0 compost, respectively). Therefore, it concluded that, obtain maximum n-3 LC-PUFA efficiency REW, an period is ideal recommended FO/ 100 whereas longer periods (48-96 h) are more suitable inclusions or compost).